RefSeq Release 229 is Now Available!

Check out RefSeq release 229, now available online and from the FTP site. You can access RefSeq data through NCBI Datasets. The release is provided in several directories as a complete dataset and also as divided by logical groupings.

What’s included in this release?

As of March 3, 2025, this full release incorporates genomic, transcript, and protein data containing:

• 522,879,448 records
• 399,577,538 proteins
• 68,985,910 RNAs
• Sequences from 164,117 organisms 

New eukaryotic genome annotations

This release contains new or updated annotations generated by NCBI’s eukaryotic genome annotation pipeline for 30 species, including:

RefSeq assembly GCF_902729225.1

The sequences associated with GCF_902729225.1 should be Pteropus medius (Taxonomy ID 143291). However, some data updates related to BioSample SAMEA5634742 were not complete by the time of this release, so the sequences are currently assigned to Pteropus vampyrus (Taxonomy ID 132908).

Missing CDD annotation

In this release, approximately 75 million proteins are missing expected CDD annotation. This issue is related to a processing error and is currently being investigated.

Upcoming changes to NCBI Taxonomy for viruses (Spring 2025)

As previously mentioned, NCBI will add binomial species names to about 3,000 viruses to reflect changes to the International Code of Virus Classification and Nomenclature (ICVCN).

Stay up to date

RefSeq is part of the NIH Comparative Genomics Resource (CGR). CGR facilitates reliable comparative genomics analyses for all eukaryotic organisms through an NCBI Toolkit and community collaboration. Follow us on social @NCBI and join our mailing list to keep up to date with RefSeq and other CGR news.

Questions?

If you have questions or would like to provide feedback, please reach out to us!